3ID4
Crystal Structure of RseP PDZ2 domain fused GKASPV peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.96386 |
Spacegroup name | P 31 |
Unit cell lengths | 35.094, 35.094, 62.373 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.766 - 1.604 |
R-factor | 0.1932 |
Rwork | 0.192 |
R-free | 0.21870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3id2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.883 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 10945 | |
<I/σ(I)> | 33.1 | 2.8 |
Completeness [%] | 97.5 | 85 |
Redundancy | 3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2M (NH4)2SO4, 30%(w/v) Polyethylene Glycol 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |