3ID2
Crystal Structure of RseP PDZ2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.961, 108.961, 58.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.817 - 3.089 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zpm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.820 | 3.200 |
| High resolution limit [Å] | 3.089 | 3.089 |
| Number of reflections | 6668 | |
| <I/σ(I)> | 19.8 | 3.24 |
| Completeness [%] | 97.6 | 90.4 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 4M Sodium Formate, 4% Methanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
