3ID2
Crystal Structure of RseP PDZ2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-24 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 108.961, 108.961, 58.335 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.817 - 3.089 |
R-factor | 0.2078 |
Rwork | 0.205 |
R-free | 0.26980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zpm |
RMSD bond length | 0.011 |
RMSD bond angle | 1.409 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.820 | 3.200 |
High resolution limit [Å] | 3.089 | 3.089 |
Number of reflections | 6668 | |
<I/σ(I)> | 19.8 | 3.24 |
Completeness [%] | 97.6 | 90.4 |
Redundancy | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 4M Sodium Formate, 4% Methanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |