3ID1
Crystal Structure of RseP PDZ1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-05 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.740, 59.740, 61.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.870 - 1.670 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23620 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.847 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.770 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.390 | |
| Total number of observations | 89799 | |
| Number of reflections | 14152 | |
| <I/σ(I)> | 26.9 | 3.2 |
| Completeness [%] | 93.1 | 93.1 |
| Redundancy | 5.91 | 1.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100mM Bis-tris propane, pH7.0, 2.5M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
