3ICY
The crystal structure of sensory box histidine kinase/response regulator domain from Chlorobium tepidum TLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.728, 32.483, 93.755 |
| Unit cell angles | 90.00, 114.67, 90.00 |
Refinement procedure
| Resolution | 26.917 - 2.684 |
| R-factor | 0.1711 |
| Rwork | 0.167 |
| R-free | 0.24830 |
| Structure solution method | SAD |
| Starting model (for MR) | ARP/wARP |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.086 | 0.260 |
| Number of reflections | 8394 | |
| <I/σ(I)> | 27.6 | 7 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.5M (NH4)2HPO4, 10% PEG400, Na-citrate, pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
