3IC9
The structure of dihydrolipoamide dehydrogenase from Colwellia psychrerythraea 34H.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97937, 0.97953 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.497, 246.353, 73.664 |
Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
Resolution | 43.900 - 2.150 |
R-factor | 0.1885 |
Rwork | 0.186 |
R-free | 0.23338 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.427 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.107 | 0.460 |
Number of reflections | 138959 | |
<I/σ(I)> | 17.6 | 2.2 |
Completeness [%] | 99.1 | 93.6 |
Redundancy | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1M Bis-tris, 20%(w/v) PEG MME5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |