3IC7
Crystal Structure of Putative Transcriptional Regulator of GntR Family from Bacteroides thetaiotaomicron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 82.826, 82.826, 85.162 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.240 - 2.819 |
R-factor | 0.237 |
Rwork | 0.236 |
R-free | 0.26300 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.387 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.4_58)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.240 | 2.870 |
High resolution limit [Å] | 2.819 | 2.820 |
Number of reflections | 8473 | |
<I/σ(I)> | 8.3 | 2 |
Completeness [%] | 99.7 | 96.7 |
Redundancy | 11.5 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 45 % MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298K |