3IC6
Crystal structure of putative methylase family protein from Neisseria gonorrhoeae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.123, 73.123, 193.315 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.590 |
R-factor | 0.21039 |
Rwork | 0.209 |
R-free | 0.24433 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.496 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.620 |
High resolution limit [Å] | 2.590 | 2.600 |
Rmerge | 0.103 | 0.570 |
Number of reflections | 10136 | |
<I/σ(I)> | 40.1 | 4.76 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 9.9 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 8% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |