3IC4
The crystal structure of the glutaredoxin(grx-1) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.951, 45.206, 45.461 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.060 - 1.700 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23865 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.667 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.400 | 1.744 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.064 | 0.311 |
Number of reflections | 10091 | |
<I/σ(I)> | 30.58 | 2.13 |
Completeness [%] | 99.4 | 97.44 |
Redundancy | 9.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M Hepes, 0.2M MgCl2, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |