3IC3
Structure of a putative pyruvate dehydrogenase from the photosynthetic bacterium Rhodopseudomonas palustrus CGA009
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97948, 0.97935 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.700, 64.995, 116.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.480 - 1.800 |
R-factor | 0.16839 |
Rwork | 0.167 |
R-free | 0.19545 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.407 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.596 |
Number of reflections | 46379 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 278 | 0.1M phosphate critrate pH 4.2 1.6M NaH2PO4, 0.4M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 278K |