3IBZ
Crystal structure of putative tellurium resistant like protein (TerD) from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.836, 61.456, 85.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.940 - 1.780 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.20000 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.789 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.940 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.071 | 0.554 |
| Number of reflections | 18188 | |
| <I/σ(I)> | 22.1 | 2.2 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1M TRIS PH8.5, 12% GLYCEROL, 1.5M NH4SO4, VAPOR DIFFUSION - SITTING DROP, TEMPERATURE 273K, temperature 293K |
