3IBY
Structure of cytosolic domain of L. pneumophila FeoB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.75 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.056, 130.697, 157.616 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.810 - 2.500 |
R-factor | 0.21621 |
Rwork | 0.212 |
R-free | 0.28770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.655 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.810 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.081 | 0.481 |
Number of reflections | 36133 | |
Completeness [%] | 93.2 | 68.1 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 25% tacsimate, 10% poly-L-lysine, 50 mM Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |