3IBR
Crystal Structure of P. aeruginosa Bacteriophytochrome Photosensory Core Module Mutant Q188L in the Mixed Pr/Pfr State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 65 |
| Unit cell lengths | 108.902, 108.902, 188.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.740 - 2.970 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.30700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.336 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.040 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.066 | 0.510 |
| Number of reflections | 23250 | |
| <I/σ(I)> | 10.7 | 1.3 |
| Completeness [%] | 94.2 | 80.8 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | protein: 10mg/ml, 0.5% PEG4000 (w/v), 0.01M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
