3IBP
The Crystal Structure of the Dimerization Domain of Escherichia coli Structural Maintenance of Chromosomes Protein MukB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.971976, 0.979795, 0.979656 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 56.270, 56.270, 342.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.270 - 3.099 |
| R-factor | 0.247 |
| Rwork | 0.243 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.254 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (2009_02_15_2320_3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 3.150 |
| High resolution limit [Å] | 3.099 | 3.099 |
| Rmerge | 0.095 | 0.619 |
| Number of reflections | 10802 | |
| <I/σ(I)> | 24.5 | 1.5 |
| Completeness [%] | 98.5 | 83 |
| Redundancy | 12.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 165 mM MOPS, 165 mM Ammonium Bromide, 13.5% PEG 20k, pH 6.5, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
