3IAP
E. coli (lacZ) beta-galactosidase (E416Q)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11589 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.230, 162.340, 202.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.380 - 2.000 |
| R-factor | 0.18141 |
| Rwork | 0.181 |
| R-free | 0.22042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.420 | 2.850 |
| High resolution limit [Å] | 2.000 | 2.700 |
| Rmerge | 0.149 | 0.408 |
| Number of reflections | 120198 | |
| <I/σ(I)> | 5.2 | 2.5 |
| Completeness [%] | 87.0 | 90.2 |
| Redundancy | 3.29 | 3.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | PEG8000, NaCl, MgCl2, DTT, Bis-Tris, pH6.5, HANGING DROP AT 288K, VAPOR DIFFUSION, HANGING DROP |
