3I99
The crystal structure of the UDP-N-acetylenolpyruvoylglucosamine reductase from the Vibrio cholerae O1 biovar Tor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 43 |
| Unit cell lengths | 84.334, 84.334, 53.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.080 - 2.200 |
| R-factor | 0.17087 |
| Rwork | 0.168 |
| R-free | 0.22334 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.041 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.330 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.138 | 0.750 |
| Number of reflections | 18139 | |
| <I/σ(I)> | 18.48 | 2 |
| Completeness [%] | 99.3 | 96.82 |
| Redundancy | 6.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.1M Phosphate-citrate, 1.6M NaH2PO4/0.4M K2HPO4, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
