3I92
Structure of the cytosolic domain of E. coli FeoB, GppCH2p-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.724, 55.925, 90.931 |
| Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
| Resolution | 45.450 - 3.000 |
| R-factor | 0.21942 |
| Rwork | 0.217 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.450 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 14810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 30% PEG 3350, 12.5% tacsimate, 250 mM 6-amino hexanoic acid, 100 mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
