3I8X
Structure of the cytosolic domain of E. coli FeoB, GDP-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9085 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.731, 56.058, 90.799 |
Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
Resolution | 25.140 - 2.250 |
R-factor | 0.18946 |
Rwork | 0.186 |
R-free | 0.24965 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i8s |
RMSD bond length | 0.015 |
RMSD bond angle | 1.516 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.150 |
High resolution limit [Å] | 2.250 |
Number of reflections | 35991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.9 | 285 | 10 % PEG 4000, 100 mM Na-malonate, pH 4.9, VAPOR DIFFUSION, temperature 285K |