3I8T
N-terminal CRD1 domain of mouse Galectin-4 in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 90.841, 90.841, 57.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.560 - 2.100 |
| R-factor | 0.18874 |
| Rwork | 0.187 |
| R-free | 0.22413 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gal |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.817 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.620 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.071 | 0.472 |
| Number of reflections | 14348 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.2 | 97.5 |
| Redundancy | 7.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M NaCacodylate pH5.0, 15% PEG4000, 0.2M Ammonium Sulphate, 80mM lactose, protein concentration 9.6mg/ml, drop 1+1ul, microseeding, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
