3I8S
Structure of the cytosolic domain of E. coli FeoB, nucleotide-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.1758, 0.9783, 0.9792, 0.8266 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.400, 56.280, 90.620 |
Unit cell angles | 90.00, 91.86, 90.00 |
Refinement procedure
Resolution | 23.820 - 1.800 |
R-factor | 0.17867 |
Rwork | 0.177 |
R-free | 0.21742 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.585 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.900 |
High resolution limit [Å] | 1.800 |
Number of reflections | 69615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.9 | 285 | 10 % PEG 4000, 100 mM Na-malonate, pH 4.9, VAPOR DIFFUSION, temperature 285K |