3I8B
The crystal structure of xylulose kinase from Bifidobacterium adolescentis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.732, 61.918, 94.606 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 48.298 - 2.000 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.094 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.130 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.159 | 0.532 |
| Number of reflections | 37936 | |
| <I/σ(I)> | 10.9 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.2 M Ammonium acetate, 0.1 M sodium acetate trihydrate pH 4.6, 30% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
