3I87
Ethanolamine Utilization Microcompartment Shell Subunit, EutL Open Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 67.799, 67.799, 80.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 80.580 - 2.300 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.873 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.131 | 0.048 | 0.493 |
Number of reflections | 8975 | ||
<I/σ(I)> | 7.5 | ||
Completeness [%] | 90.9 | 84.7 | 93.6 |
Redundancy | 6 | 6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M sodium/potassium phosphate, 2.2M sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |