3I82
Ethanolamine Utilization Microcompartment Shell Subunit, EutL Closed Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 67.260, 67.260, 79.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.810 - 2.310 |
| R-factor | 0.20782 |
| Rwork | 0.206 |
| R-free | 0.24378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i87 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.275 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.167 | 0.066 | 0.576 |
| Number of reflections | 9144 | ||
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 94.7 | 89.6 | 97.1 |
| Redundancy | 9.5 | 9.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M sodium/potassium phosphate, 2.5M sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
