3I7T

Crystal structure of Rv2704, a member of highly conserved YjgF/YER057c/UK114 family, from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-19
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 3
Unit cell lengths	67.880, 67.880, 67.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.930
R-factor	0.20686
Rwork	0.205
R-free	0.24678
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.241
Data reduction software	MOSFLM (3.3.9)
Data scaling software	SCALA (3.3.9)
Phasing software	SHELXS
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.880	39.190	2.030
High resolution limit [Å]	1.930	6.100	1.930
Rmerge		0.041	0.424
Total number of observations		4732	20323
Number of reflections	8092
<I/σ(I)>	21.5	16.1	6.6
Completeness [%]	100.0	99.6	100
Redundancy	17.8	16.3	17.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	295	15% PEG8000, 0.1M Bis Tris, pH6.5, Microbatch, under-oil, temperature 295K