3I7H
Crystal Structure of DDB1 in Complex with the H-Box Motif of HBX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-05-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0,1.005 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.856, 132.860, 183.818 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.900 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5m |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.101 | 0.378 |
| Number of reflections | 31592 | |
| <I/σ(I)> | 24.2 | 3.8 |
| Completeness [%] | 95.7 | 70 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16% PEG 4000, 0.2M SODIUM CHLORIDE, 0.1M MES, 0.005M DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
