3I79
Calcium-Dependent Protein Kinase 1 from Toxoplasma gondii (TgCDPK1)
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-22 |
Detector | MarUSA MarMosaic -325 CCD |
Wavelength(s) | 0.97923 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.144, 71.861, 67.265 |
Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
Resolution | 36.890 - 2.040 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.25800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.866 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | REFMAC (refmac_5.5.0096) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.052 | 0.670 |
Number of reflections | 28324 | |
<I/σ(I)> | 10.4 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 6.5 | 298 | 25% PEG 3350, 0.2 M tri-ammonium citrate; cryoprotected with 15% ethylene glycol, pH 6.5, sitting drop vapor diffusion, temperature 298K |
1 | sitting drop vapor diffusion | 6.5 | 298 | 25% PEG 3350, 0.2 M tri-ammonium citrate; cryoprotected with 15% ethylene glycol, pH 6.5, sitting drop vapor diffusion, temperature 298K |
1 | sitting drop vapor diffusion | 6.5 | 298 | 25% PEG 3350, 0.2 M tri-ammonium citrate; cryoprotected with 15% ethylene glycol, pH 6.5, sitting drop vapor diffusion, temperature 298K |