3I77
35/99/170-loops of FXa in SGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 138.351, 138.351, 81.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.080 - 2.100 |
| R-factor | 0.16429 |
| Rwork | 0.163 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1os8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.080 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.162 | 0.624 |
| Number of reflections | 21801 | |
| <I/σ(I)> | 14.4 | 3.8 |
| Completeness [%] | 98.9 | |
| Redundancy | 10.2 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.5 M Ammonium sulfate, 10 mM Calcium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
