3I71

Ethanolamine Utilization Microcompartment Shell Subunit, EutK C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9717
Spacegroup name	P 61 2 2
Unit cell lengths	65.345, 65.345, 146.772
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.810 - 2.100
R-factor	0.207
Rwork	0.206
R-free	0.23000
Structure solution method	SIRAS
RMSD bond length	0.015
RMSD bond angle	1.435
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.070	0.045	0.326
Number of reflections	11489
<I/σ(I)>	13.4
Completeness [%]	99.9	99.3	100
Redundancy	19.7	17.4	20.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	0.1M sodium citrate, 20.4% PEG4000, 16% isopropanol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K