3I6L
Newly identified epitope N1 derived from SARS-CoV N protein complexed with HLA-A*2402
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-12 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.422, 85.823, 92.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.940 - 2.400 |
| R-factor | 0.21669 |
| Rwork | 0.213 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bck |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.940 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.099 | 0.387 |
| Total number of observations | 149410 | |
| Number of reflections | 22272 | |
| <I/σ(I)> | 40.671 | 6.211 |
| Completeness [%] | 98.6 | 97.5 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 277 | 0.2M Ammonium sulfate, 0.1M Tris pH 8.9, 25% w/v Polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
