3I69
Apo Glutathione Transferase A1-1 GIMF-helix mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.896, 114.684, 97.538 |
| Unit cell angles | 90.00, 117.76, 90.00 |
Refinement procedure
| Resolution | 47.220 - 2.380 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.28900 |
| Structure solution method | isomorphous to another structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | structure (factor calculator for an isomorphous structure) |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.750 |
| Number of reflections | 83015 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.9 | 94.7 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 18% PEG 4000, 0.1 M Tric-Cl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
