3I5Y
Structure of Mss116p bound to ssRNA containing a single 5-BrU and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-16 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.91842 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.190, 126.352, 55.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.880 - 2.490 |
| R-factor | 0.20573 |
| Rwork | 0.204 |
| R-free | 0.24206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i5x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.073 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.5.0070) |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | 0.426 |
| Number of reflections | 21524 | |
| <I/σ(I)> | 25.1 | 3.5 |
| Completeness [%] | 94.5 | 75.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 8% tacsimate, 15% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
