3I5X
Structure of Mss116p bound to ssRNA and AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2008-10-16 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.91842 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.540, 126.524, 55.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.000 - 1.900 |
R-factor | 0.19229 |
Rwork | 0.191 |
R-free | 0.22480 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.077 | 0.389 |
Number of reflections | 48438 | |
<I/σ(I)> | 30.6 | 4.9 |
Completeness [%] | 96.4 | 82.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.2 M succinate, 15% PEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |