3I5V
Crystal structure of beta toxin 275-280 from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90020 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.184, 69.053, 75.654 |
| Unit cell angles | 93.06, 94.60, 92.13 |
Refinement procedure
| Resolution | 38.400 - 2.800 |
| R-factor | 0.2141 |
| Rwork | 0.214 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Wild-type beta toxin from Staphylococcus aureus |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 2.990 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 22252 | |
| Completeness [%] | 95.0 | 38.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.15-0.25 M NaF, 28-34% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
