3I5C
Crystal structure of a fusion protein containing the leucine zipper of GCN4 and the GGDEF domain of WspR from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.813, 92.813, 133.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.868 - 1.940 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 43941 | |
| <I/σ(I)> | 55.1 | 4 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 18.1 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Magnesium formate dihydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
