3I57

Type 2 repeat of the mucus binding protein MUB from Lactobacillus reuteri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-10-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	44.861, 45.846, 191.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.620 - 1.800
R-factor	0.206
Rwork	0.204
R-free	0.25800
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.351
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	40.620
High resolution limit [Å]	1.800
Number of reflections	36176
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.2 M ammonium formate, 22 % (w/v) PEG 3350, unbuffered, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K