3I50

Crystal structure of the West Nile Virus envelope glycoprotein in complex with the E53 antibody Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-06
Detector	NOIR-1
Wavelength(s)	1.240
Spacegroup name	P 21 21 2
Unit cell lengths	146.410, 160.140, 43.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
Rwork	0.247
R-free	0.34400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.858
Data reduction software	d*TREK
Data scaling software	d*TREK (9.4D)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.150	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.053	0.453
Number of reflections	21428
<I/σ(I)>	12.1	2
Completeness [%]	99.5	99.8
Redundancy	4.45	4.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	30% PEG 300, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K