3I4P
Crystal structure of AsnC family transcriptional regulator from Agrobacterium tumefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-14 |
Detector | ARGONNE APS1 CCD |
Wavelength(s) | 0.9767 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 141.833, 141.833, 50.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.21400 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.526 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.145 | 0.980 |
Number of reflections | 13438 | |
<I/σ(I)> | 22.89 | 2.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 12.9 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 273 | 0.1M NA(OAC), 3.2M NACL, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 273K |