3I4I
Crystal structure of a prokaryotic beta-1,3-1,4-glucanase (lichenase) derived from a mouse hindgut metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.367, 79.804, 85.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.670 - 1.890 |
| R-factor | 0.17267 |
| Rwork | 0.170 |
| R-free | 0.21549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1byh |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.670 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmerge | 0.060 | 0.515 |
| Number of reflections | 43509 | |
| <I/σ(I)> | 18.6 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M SPG buffer pH 8.0, 25% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
