3I4H
Crystal structure of Cas6 in Pyrococcus furiosus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 18-ID |
| Synchrotron site | APS |
| Beamline | 18-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.5418,1.5621,1.7321 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.745, 84.745, 81.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.500 - 2.250 |
| R-factor | 0.22951 |
| Rwork | 0.226 |
| R-free | 0.28896 |
| Structure solution method | SAD |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.500 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| <I/σ(I)> | 2.4 | 2.4 |
| Completeness [%] | 89.0 | 48 |
| Redundancy | 17.7 | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 303 | 0.02 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 6.0, 15% v/v 2-Propanol, temperature 303K, VAPOR DIFFUSION, HANGING DROP |
