3I4E
Crystal structure of Isocitrate Lyase from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Collection date | 2008-06-19 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.666, 137.025, 160.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.910 - 2.690 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.019 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.700 |
| Rmerge | 0.144 | 0.660 |
| Number of reflections | 48029 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 6.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 293 | 24% PEG 1500, 100 MM MES, PROTEIN CONCENTRATION 22MG/ML, PH 5.8, VAPOR DIFFUSIONI, SITTING DROP, TEMPERATURE 293K, VAPOR DIFFUSION, SITTING DROP |
