3I45
CRYSTAL STRUCTURE OF putative twin-arginine translocation pathway signal protein from Rhodospirillum rubrum Atcc 11170
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.473, 91.835, 48.166 |
| Unit cell angles | 90.00, 106.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.360 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.17400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.380 |
| High resolution limit [Å] | 1.360 | 3.690 | 1.360 |
| Rmerge | 0.039 | 0.027 | 0.309 |
| Number of reflections | 156188 | ||
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 99.2 | 92.9 | 98.8 |
| Redundancy | 2.1 | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 8000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
