3I42
Structure of response regulator receiver domain (CheY-like) from Methylobacillus flagellatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.998, 81.998, 33.291 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.510 - 2.150 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.104 | 0.061 | 0.587 |
| Number of reflections | 7121 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 99.4 | 99.5 | 95.1 |
| Redundancy | 9.8 | 9.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Tris HCl pH 7.0, 0.2M NaCl, 1M Na-citrate dihydrate, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
