3I3Y
Crystal structure of Ribokinase in Complex with D-Ribose from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.488, 105.900, 121.152 |
| Unit cell angles | 90.00, 99.45, 90.00 |
Refinement procedure
| Resolution | 34.970 - 2.150 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.150 | 2.040 |
| Number of reflections | 84799 | |
| <I/σ(I)> | 8.1 | 3.8 |
| Completeness [%] | 99.5 | 95.2 |
| Redundancy | 7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 0.2M LITHIUM SULFATE MONO HYDRATE, 0.1M HEPES pH 7.5, 20% PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
