3I3S
Crystal Structure of H-Ras with Thr50 replaced by Isoleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9998 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 89.210, 89.210, 134.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.860 - 1.360 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.400 | |
| High resolution limit [Å] | 1.360 | 25.000 | 1.360 |
| Rmerge | 0.043 | 0.036 | 0.295 |
| Number of reflections | 44357 | 4 | 3653 |
| <I/σ(I)> | 25.36 | 57.2 | 4.6 |
| Completeness [%] | 99.8 | 30.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 285 | 17% PEG 6000, 200 mM CaCl2, pH 7.5, hanging drop, temperature 285K |
