3I3G
Crystal Structure of Trypanosoma brucei N-acetyltransferase (Tb11.01.2886) at 1.86A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.860, 64.440, 82.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.960 - 1.860 |
| R-factor | 0.2454 |
| Rwork | 0.243 |
| R-free | 0.28170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fb3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.960 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.049 | 0.237 |
| Number of reflections | 23625 | |
| <I/σ(I)> | 25.5 | 7.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19% PEG3350, 0.2M (NH4)2Tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
