3I3C
Crystal Structural of CBX5 Chromo Shadow Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.660, 126.587, 31.399 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.480 |
| R-factor | 0.268 |
| Rwork | 0.266 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.676 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.590 |
| High resolution limit [Å] | 2.480 | 5.380 | 2.480 |
| Rmerge | 0.093 | 0.093 | 0.240 |
| Number of reflections | 11761 | ||
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 97.1 | 97.6 | 83.9 |
| Redundancy | 6.4 | 6.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20% PEG3350, 0.2M di-Na Tartrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
