3I32
Dimeric structure of a Hera helicase fragment including the C-terminal RecA domain, the dimerization domain, and the RNA binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-18 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.960, 64.960, 153.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.420 - 2.800 |
R-factor | 0.2553 |
Rwork | 0.253 |
R-free | 0.29250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Hera RBD and 2eaq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.155 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.420 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.081 | 0.911 |
Number of reflections | 12078 | |
<I/σ(I)> | 28.2 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 50 | 51.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 0.1 M MES-NaOH pH 6.5, 15-18% ethylene glycol, 0.3-0.5 M NaCl, 3-6% PEG 20 000, VAPOR DIFFUSION, temperature 277K |