3I31
Hera helicase RNA binding domain is an RRM fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-06-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 37.516, 37.516, 136.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.222 - 1.800 |
R-factor | 0.2015 |
Rwork | 0.200 |
R-free | 0.22350 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.202 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | SHELXDE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.180 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 9812 | |
<I/σ(I)> | 7.5 | 1.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M NaCac pH 6.5, 16-18% P8000, 0.3-0.4M Zn(OAc)2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M NaCac pH 6.5, 16-18% P8000, 0.3-0.4M Zn(OAc)2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |