3I2V
Crystal structure of human MOCS3 rhodanese-like domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0720 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.817, 31.911, 50.640 |
| Unit cell angles | 90.00, 110.16, 90.00 |
Refinement procedure
| Resolution | 24.258 - 1.250 |
| R-factor | 0.1614 |
| Rwork | 0.159 |
| R-free | 0.20430 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.260 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.617 | |
| Number of reflections | 30819 | |
| <I/σ(I)> | 33.7 | 2.83 |
| Completeness [%] | 90.7 | 66.3 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES pH 7.5, 21% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
