3I2N
Crystal Structure of WD40 repeats protein WDR92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-22 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 |
| Unit cell lengths | 70.052, 70.052, 84.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.180 - 1.950 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.763 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.070 | 0.034 | |
| Number of reflections | 29854 | ||
| <I/σ(I)> | 14.1 | ||
| Completeness [%] | 99.8 | 99.5 | 98.4 |
| Redundancy | 7.1 | 7.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.35M Na/K phosphate, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
