3I2M
The Crystal Structure of PF-8, the DNA Polymerase Accessory Subunit from Kaposi s Sarcoma-Associated Herpesvirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Detector technology | CCD |
Collection date | 2008-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .97918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 58.300, 58.300, 386.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.150 - 2.810 |
R-factor | 0.25311 |
Rwork | 0.252 |
R-free | 0.28131 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.839 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.095 | 0.492 |
Number of reflections | 10103 | |
<I/σ(I)> | 18.012 | 2.7 |
Completeness [%] | 95.2 | 62.1 |
Redundancy | 11.1 | 0.049 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 295 | 18% PEG 3350, 100 mM Tris HCl pH 8.2, 0.2 M lithium chloride, and 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |